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The Super Mario All-Stars version of Super Mario Bros. has unused debug functionality to replace Mario's sprite with a blue rectangle reading "PLAYER". The rectangle also becomes red when Mario turns into Fire Mario.
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In Donkey Kong Country 2, if Dixie lands on Rambi while performing a helicopter spin and B is pressed on the next frame while Y continues being held, the properties of the helicopter spin will be transferred onto Rambi, allowing him to float through the air.
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In World 2-1 of Super Mario Bros., letting too many enemies gather near the final pipe of the level will cause entities to start despawning, which may cause the trampoline to disappear. Jumping into the space where it was will trap Mario in mid-air forever.
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In Super Mario Bros., any moving platform that scrolls offscreen so that less than 8 pixels of it remain is no longer displayed despite still being loaded. Standing on such a platform causes Mario to appear to float.
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In Super Mario Bros. 2, the animation of launching in a rocket ship usually takes a few seconds. By dropping a POW Block immediately after launching, the rocket's speed will increase drastically.