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In Super Mario Galaxy 2, the Starshroom appears near Starship Mario after reaching Spin-Dig Galaxy. However, even before that, one of the Toad Brigade members is already present: Banktoad can be seen floating in mid-air above Starship Mario from the beginning of the game.
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Development files for Super Mario World contain sprites for Mario moving towards and away from the camera. He does so by assuming a unique transitional state between Small Mario and Super Mario, which is shown here in the middle alongside the two standard forms.
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For the British commercial for Super Mario Sunshine, 22 seconds of live-action footage of people with Mario mustaches were prepared, but only 5 seconds were actually used in the finished version. Below is the unreleased full commercial, including the 17 seconds of deleted scenes.
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In Super Mario 64, collecting a star above an updraft will cause Mario to float in the wind indefinitely as control is taken away from the player, preventing Mario from escaping the updraft. The only actions the player can perform are moving the camera and turning off the game.
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Development files for Super Mario Advance contain voice samples of Mario, Luigi, Peach and Toad laughing in unusual, possibly intentionally odd-sounding ways. It is unknown what these clips would have been used for.
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In Super Mario 64, the floating boxes above the elevator in Hazy Maze Cave are at a precise height where, if the edges of the elevator are aligned with them, Mario can bypass them by briefly getting squished into the gap.
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In Super Mario Bros. 2, the animation of launching in a rocket ship usually takes a few seconds. By dropping a POW Block immediately after launching, the rocket's speed will increase drastically.
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In Super Mario Odyssey, there are two NPCs sitting on a bench in Bowser's Kingdom. With a precise jump, Mario can land in a spot not covered by either of their collision, allowing him to forcibly insert himself between them on the bench.
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In Paper Mario: The Thousand-Year Door, glitches can be used to let Flavio follow Mario for the entirety of the game. Scenes not originally intended to have Flavio then exhibit "Flavio omnipresence" whereby instead of physically entering the scene, he is already always there.
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In WarioWare, Inc.: Mega Microgames, there is a small chance for the third level of the "Right in the Eye" microgame to be unwinnable. The game can put the needle too low to be threaded. In the footage, Down is held the entire time; the hand will simply not go any lower.
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Super Mario 64 contains unused code for throwing MIPS the rabbit, including a unique animation of him falling onto the floor and getting back up. In the finished version of the game, this was replaced by Mario carefully setting MIPS down instead of throwing him.
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