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In World 2-1 of Super Mario Bros., letting too many enemies gather near the final pipe of the level will cause entities to start despawning, which may cause the trampoline to disappear. Jumping into the space where it was will trap Mario in mid-air forever.
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In Captain Toad: Treasure Tracker, waiting for 300 seconds in the Bizarre Doors of Boo Mansion level will cause a set of horror movie-like handprints to suddenly appear on one of the walls.
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Due to widespread disagreement over the nature of the Underwhere in Super Paper Mario and the exact mechanism behind Mario being sent there, the previous Tweet can no longer be considered factual and thus has been deleted. I apologize for any undue speculation on my part.
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In Super Mario 64, if Z is pressed on the frame Mario touches a Koopa shell, he will not ride it, and instead the background music will restart from the beginning. Note the Bob-omb Battlefield music intro replaying whenever Mario crouches in the footage.
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In Super Mario Odyssey, whenever characters dance or bob to the music, the speed of their animations will change depending on the tempo of the music track currently playing. Note the Jammin' Toad bobbing much faster when a more lively track is played.
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In Super Mario Bros., any moving platform that scrolls offscreen so that less than 8 pixels of it remain is no longer displayed despite still being loaded. Standing on such a platform causes Mario to appear to float.
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A follow-up to the recent post about the rare artwork of Toad and Yoshi holding hands: reader @_Kingboomber_ has faithfully recreated the artwork by tracing over the original scan for those who wished for a high-quality version of it. Thank you very much for your excellent work!
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Super Mario Galaxy 2 contains a piece of music the game considers forbidden: the Yoshi version of the "Forest Dwellers" theme. Whenever Yoshi would enter an area where "Forest Dwellers" plays, instead of playing this version, the game crashes.