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For the US commercial for Mario & Luigi: Superstar Saga, 48 seconds of live-action footage of child/adolescent Mario and Luigi were prepared, but only 21 were actually used in the finished version. Below is the entirety of the footage, including the 27 seconds of deleted scenes.
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In Super Mario Galaxy, there is a piece of faulty collision in front of the door to the Garden on the Comet Observatory that will cause Mario to enter a variety of glitched animations based on his position, some of which are showcased below.
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A brief announcement: since I have received many inquiries regarding the Supper Mario Broth carrot Mii, here is a close-up look of it and instructions on how to recreate it, courtesy of renowned Mii expert @HEYimHeroic. Regular content will now resume.
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On the title screen of Mario Paint, clicking the "R" plays a sound. That sound is actually a voice saying "Odnetnin", or "Nintendo" backwards. The footage below first plays the clip normally, then reverses it, and finally slows it down to hear the original "Nintendo" voice.
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In Super Mario Odyssey, if A is pressed on a specific frame while climbing this ledge in the Cap Kingdom, Mario will be stuck walking in place forever and not accept any further input, forcing the game to be reset.