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In Level 3-1 of Super Mario Bros. 2, there is a shortcut beyond a gap that Luigi and Peach can clear easily, but Mario and Toad can't. However, even they can access it: the decorative clouds have secret functionality where they can support Mushroom Blocks to bridge the gap.
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An audio glitch exists in the original Japanese version of Super Mario Sunshine that is fixed in all other versions. Whenever Mario enters Delfino Plaza during the flood event for the second or any subsequent time, he will let out an extremely drawn-out and distorted scream.
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In Super Mario 64, Mario's health is not restored when entering a course. By precisely timing fire damage, it is possible to enter Lethal Lava Land with 0 health. Mario will play the entering animation as normal, but die immediately upon touching the ground.
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